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2X Hot-Start PCR Master Mix

200 reactions of 50 µl

2X Hot-Start PCR Master Mix
  • Package Size: 200 reactions of 50 µl
  • Transport conditions: Mražené na suchém ledu (dry ice)
  • Storage: -20°C
  • Price without VAT: 114,00 €
  • Price including VAT: 137,94 €
114,00 €

Features

  • Highest PCR sensitivity without prolonged reactivation
  • Optimized PCR Master Mix for minimal hands-on and fast setup
  • Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs

Applications

  • High-specificity and high-throughput hot-start PCR up to 3 kb
  • Amplification of low-copy-number targets
  • TA cloning

 

Product Description:

biotechrabbit™ Hot Start PCR Master Mix is a perfect choice for a fast reaction setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for low-background, high-throughput PCR of 0.2–3 kb DNA targets.

The 2x Hot Start PCR Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added.

The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes primer–dimers and mispriming.

Info: Recommended annealing temperature is 2°C above Tm of primers or use gradient PCR to optimize the annealing temperature.

 

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