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YourTaq™ Hot-Start DNA Polymerase, 5 U/µl

250 U

YourTaq™ Hot-Start DNA Polymerase, 5 U/µl
  • Package Size: 250 U
  • Transport conditions: Mražené na suchém ledu (dry ice)
  • Storage: -20°C
Price on Request

Features

  • Exceptionally pure YourTaq Hot Start DNA Polymerase
  • Optimized buffer composition for increased yield of amplification
  • Resistant to PCR inhibitor carry-over
  • Excellent PCR specificity and sensitivity for a broad range of amplicons

Applications

  • Routine and demanding PCR amplification up to 3 kb
  • Suitable for amplification of low target copy number
  • RT-PCR and TA cloning

Product Description:

biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.

YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5X PCR Enhancer (BR1900201).

Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).

 

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