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2X YourTaq™ Hot-Start PCR Master Mix

1000 reactions of 50 µl

2X YourTaq™ Hot-Start PCR Master Mix
  • Package Size: 1000 reactions of 50 µl
  • Transport conditions: Mražené na suchém ledu (dry ice)
  • Storage: -20°C
Price on Request

Features

  • Exceptionally pure YourTaq Hot Start DNA Polymerase
  • Optimized Master Mix for increased yield of amplification
  • Resistant to PCR inhibitor carry-over
  • Excellent PCR specificity and sensitivity for a broad range of amplicons

Applications

  • Routine and demanding PCR amplification up to 3 kb
  • Suitable for amplification of low target copy number
  • TA cloning

Product Description:

biotechrabbit™ YourTaq Hot Start PCR Master Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start PCR Master Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.

The 2X YourTaq Hot Start PCR Master Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5X PCR Enhancer (BR1900201).

Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).

 

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